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Compound

Ammonium nitrogen

The nitrogen form yeast takes up fastest and the one most nutrient additions supply, useful for rescuing a ferment and a poor substitute for a properly balanced juice.

Also called Ammonium, DAP, Diammonium phosphate.

Class
Nitrogen compounds
Formula
Not a single molecule — see below
How often it matters
Regularly encountered

What it does in cider

Measured figures

Shown as they were measured, with the context each was taken in. They are not averaged: a concentration recorded in one country's fruit in one decade is not a constant.

Ammonium nitrogen8.0–9.0 mg/L

Virginia, United States, 2014–2015 · 108 samples · Enzymatic ammonia assay (Ammonia-Rapid) · Boudreau, Peck, O’Keefe and Stewart, Food Science & Nutrition 6(1):119–123

Recorded as a range because the paper itself gives two figures: the abstract says 9 mg N/L and the results section says 8 ± 1 mg/L, which is rounding rather than disagreement. Either way it is about a seventh of the assimilable nitrogen, and it did not correlate with the total — which is why the fraction yeast reaches for first is also the fraction an apple juice is least likely to have.

The inorganic fraction of assimilable nitrogen, taken up first and exhausted first. Measured in milligrams per litre.

What forms it

Processes that put this compound into the drink, or increase how much of it is there.

What removes or limits it

Processes that reduce it, hold it below a threshold, or stop it forming in the first place.

Faults it is implicated in

Being implicated is not the same as being a fault. Several of the compounds on this site are ordinary constituents of a sound cider and define a named fault only above a concentration.

Organisms that produce it

Which organism is responsible usually decides whether the compound is a feature or a symptom.

Nitrogen and sulphide are not a straight lineTwo yeast strains fermenting one apple juice at three nitrogen levels. The middle level produced the most hydrogen sulphide; one strain produced none at any level.0100200300123.750Low22.3 mg/L288.250Intermediate144.3 mg/L44.1250High369.3 mg/Lµg H₂S per 100 mLDiammonium phosphate addedStrain UCD522Strain UCD932 — none detectedBase juice 63.7 mg N/L, fermented at 20 °C
Two yeast strains fermenting one apple juice at three nitrogen levels. The middle level produced the most hydrogen sulphide; one strain produced none at any level.
Described in full
Shape
A grouped bar chart. Three groups along the bottom are the three diammonium phosphate treatments — low at 22.3, intermediate at 144.3 and high at 369.3 milligrams per litre, all added to one juice that already carried 63.7 milligrams of assimilable nitrogen per litre. Each group holds two bars, one per yeast strain, and each bar is labelled with its value.
Strain UCD522
Low 123.75, intermediate 288.25, high 44.125 micrograms of hydrogen sulphide per 100 millilitres. The intermediate treatment produced twice what the low one did and more than six times what the high one did.
Strain UCD932
Zero at all three treatments. No hydrogen sulphide was detected from this strain regardless of how much nitrogen it was given, so its three bars are drawn as a flat line at the axis and labelled “none detected”.
The threshold line
A dashed horizontal reference is not drawn, because the sensory threshold — about 0.41 micrograms per litre — is roughly a thousandth of the smallest bar here and would sit indistinguishably on the axis. That is the point worth taking away: every one of these fermentations was far above the concentration a nose can find.
What it corrects
The common rule is that low nitrogen causes sulphide and adding nutrient cures it. On these data, adding some nutrient was worse than adding none, and adding a lot was better than either — but only for the strain that made sulphide at all. Strain choice came before nitrogen.
Scope
One juice, one temperature — 20 degrees Celsius — one pitching rate, two strains, three treatments, in triplicate. The shape of the relationship is the finding; the numbers belong to this experiment.

About Ammonium nitrogen

Ammonium is the simplest nitrogen source a yeast can have, requiring no deamination and no transport machinery beyond a dedicated permease, and yeast takes it up in preference to amino acids. That is what makes diammonium phosphate the standard fermentation nutrient: it is cheap, it is unambiguous, and its effect on a sluggish ferment is quick enough to be visible.

It is also what makes it a partial answer. A cider juice deficient in assimilable nitrogen is usually deficient in other things too — thiamine most commonly, and the sterols and unsaturated fatty acids a yeast needs for membrane integrity. Ammonium supplies none of these, so a ferment fed ammonium alone can pick up speed and still finish badly. Complex nutrient preparations, based on inactivated yeast, supply the rest, which is why most modern practice uses a combination.

Timing matters more than quantity. Nitrogen added at pitching builds population; nitrogen added around a third of the way through sugar depletion supports the cells that already exist through the difficult end of the ferment. A single large early addition is used inefficiently, drives excess biomass and heat, and leaves residual nitrogen in the finished cider for spoilage organisms to find. There is a further reason for restraint: yeast excretes urea when nitrogen is abundant, and urea reacts with ethanol to form ethyl carbamate.

Related compounds

Compounds it is formed from, converted into, confused with, or routinely met alongside.

What people ask next

Questions readers ask about the things this page mentions. Each one goes to the section that answers it rather than to a page written to receive the question.

Where to go next

Sources

What this page rests on. Where a source is marked as registered rather than read, CiderHQ is recording that the body is authoritative on the subject without claiming to have worked through the document itself. See our evidence policy for what each state means.