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Compound

Hydrogen sulphide

The rotten-egg gas a nitrogen-starved yeast produces, detectable at concentrations too small to measure easily, and removable only if it is caught before it becomes something worse.

Also called H2S, Rotten egg gas.

Class
Sulphur compounds
Formula
H2S
How often it matters
Regularly encountered

What it does in cider

How it is perceived

What the compound registers as, and at roughly what concentration. Perception is not a property of the molecule alone: sugar, tannin and carbonation all change where a threshold falls.

On threshold

One of the lowest detection thresholds of any compound in fermented drinks, in the region of a microgram per litre, which is why it is smelled long before any analysis would find it.

Descriptors it is responsible for

Sensory records that name Hydrogen sulphide as a cause. Each states the perception and the mechanism behind it.

The structure it moves

Structural dimensions this compound contributes to. Direction and amount depend on concentration and on what else is in the drink; the dimensions themselves are set out in full under Sensory.
DimensionWhat it is
Fermentation characterAromas made by the ferment rather than carried in from the fruit.
FreshnessWhether the drink smells and tastes of live fruit or of time and air.

Measured figures

Shown as they were measured, with the context each was taken in. They are not averaged: a concentration recorded in one country's fruit in one decade is not a constant.

Aroma detection threshold0.41 µg/L

context not recorded · Sensory threshold quoted in a cider fermentation study, stated as >0.00041 mg/L · Song, Gibney, Cheng, Liu and Peck, Frontiers in Microbiology 11:1264

Four hundred nanograms in a litre. That is why a compound present in quantities no gravity reading would ever notice can be the first thing anybody says about a cider. The paper states the threshold rather than measuring it, and thresholds are matrix-dependent — sugar, tannin and carbonation all change what a nose finds — so treat it as the order of magnitude rather than a number to compare a batch against.

The concentration at which a compound becomes perceptible — which is why a trace compound can matter more than an abundant one. Measured in micrograms per litre.

Fermentation duration24–192 h

Ithaca, New York, United States, 2016 · Sulphide detection tubes on fermentations at 20 °C, strain UCD522 · Song, Gibney, Cheng, Liu and Peck, Frontiers in Microbiology 11:1264

The window in which sulphide was released at all. It began 24 hours after inoculation in the high-nitrogen treatment and 72 hours in the low and intermediate ones, and had stopped in every case by 192 hours — so a cider that smells of sulphide in the second week is describing something that happened in the first.

How long a stage of fermentation took under stated conditions. Never a prediction — the same juice at another temperature behaves differently. Measured in hours.

What forms it

Processes that put this compound into the drink, or increase how much of it is there.

What removes or limits it

Processes that reduce it, hold it below a threshold, or stop it forming in the first place.

Faults it is implicated in

Being implicated is not the same as being a fault. Several of the compounds on this site are ordinary constituents of a sound cider and define a named fault only above a concentration.

Organisms that produce it

Which organism is responsible usually decides whether the compound is a feature or a symptom.

Nitrogen and sulphide are not a straight lineTwo yeast strains fermenting one apple juice at three nitrogen levels. The middle level produced the most hydrogen sulphide; one strain produced none at any level.0100200300123.750Low22.3 mg/L288.250Intermediate144.3 mg/L44.1250High369.3 mg/Lµg H₂S per 100 mLDiammonium phosphate addedStrain UCD522Strain UCD932 — none detectedBase juice 63.7 mg N/L, fermented at 20 °C
Two yeast strains fermenting one apple juice at three nitrogen levels. The middle level produced the most hydrogen sulphide; one strain produced none at any level.
Described in full
Shape
A grouped bar chart. Three groups along the bottom are the three diammonium phosphate treatments — low at 22.3, intermediate at 144.3 and high at 369.3 milligrams per litre, all added to one juice that already carried 63.7 milligrams of assimilable nitrogen per litre. Each group holds two bars, one per yeast strain, and each bar is labelled with its value.
Strain UCD522
Low 123.75, intermediate 288.25, high 44.125 micrograms of hydrogen sulphide per 100 millilitres. The intermediate treatment produced twice what the low one did and more than six times what the high one did.
Strain UCD932
Zero at all three treatments. No hydrogen sulphide was detected from this strain regardless of how much nitrogen it was given, so its three bars are drawn as a flat line at the axis and labelled “none detected”.
The threshold line
A dashed horizontal reference is not drawn, because the sensory threshold — about 0.41 micrograms per litre — is roughly a thousandth of the smallest bar here and would sit indistinguishably on the axis. That is the point worth taking away: every one of these fermentations was far above the concentration a nose can find.
What it corrects
The common rule is that low nitrogen causes sulphide and adding nutrient cures it. On these data, adding some nutrient was worse than adding none, and adding a lot was better than either — but only for the strain that made sulphide at all. Strain choice came before nitrogen.
Scope
One juice, one temperature — 20 degrees Celsius — one pitching rate, two strains, three treatments, in triplicate. The shape of the relationship is the finding; the numbers belong to this experiment.

About Hydrogen sulphide

Yeast needs sulphur to make cysteine and methionine, and it gets it by taking sulphate from the juice and reducing it, step by step, to sulphide. The sulphide is then supposed to be joined immediately to a nitrogen-containing precursor. If the yeast has no nitrogen to spare, the sulphide has nowhere to go, and it leaves the cell as hydrogen sulphide. The smell of a ferment going wrong is, in the most literal sense, the smell of a yeast that has run out of nitrogen halfway through a biosynthetic pathway.

This is why the fault is so much more common in cider than in wine. Apple juice is chronically short of assimilable nitrogen; grape must usually is not. A cider ferment that produces hydrogen sulphide is not unlucky, it is under-fed, and the fault is better understood as a nutritional symptom rather than a hygiene one.

Timing decides whether it can be fixed. As free gas it is volatile and reactive: racking with a splash, stirring, or a small copper addition will remove it, and the cider recovers completely. Left in contact with the cider, it reacts with ethanol and other components to form mercaptans, and mercaptans oxidise to disulphides. Neither responds well to the treatments that work on the gas, and disulphides are effectively permanent. A ferment that smells of rotten egg should be dealt with in days, not weeks.

The genuine solution is upstream. Measuring assimilable nitrogen before pitching, and feeding the ferment at the right point rather than after the smell appears, prevents the whole sequence. It is also worth noting the alternative source: elemental sulphur used as an orchard fungicide can carry into juice on the fruit and be reduced by yeast in exactly the same way, which is one of the few places where a spray programme reaches directly into a fermentation.

Related compounds

Compounds it is formed from, converted into, confused with, or routinely met alongside.

What people ask next

Questions readers ask about the things this page mentions. Each one goes to the section that answers it rather than to a page written to receive the question.

Where to go next

Sources

What this page rests on. Where a source is marked as registered rather than read, CiderHQ is recording that the body is authoritative on the subject without claiming to have worked through the document itself. See our evidence policy for what each state means.